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SMeagol is a software suite for simulation of live cell single molecule microscopy, mainly aimed at single particle tracking applications. The code uses a combination of Matlab and compiled C++ executables, and runs on linux, windows and mac. Installation instructions, system requirements, and many more details can be found in the manual.
If you use SMeagol for in your research, please cite
Lindén, M., V. Ćurić, A. Boucharin, D. Fange, and J. Elf. Simulated single molecule microscopy with SMeagol. Bioinformatics. 32: 2394-2395 (2016).
Full text at http://dx.doi.org/10.1093/bioinformatics/btw109 (open access) or http://arxiv.org/abs/1509.04031 (pre-print).
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