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SpikeAnalyzer is a semiautomatic program for analysis of calcium spikes in cardiac myocytes created in the MATLAB environment. The analysis parameters are automatically initialized upon loading the electrophysiological and confocal data in their respective proprietary formats (confocal data - .tif, electrophysiological data – .abf ver. 2.0). The analysis starts with interactive selection of spike positions and determining their temporal fluorescence profiles. The data approximation uses an automatic fitting procedure with visual control over the process and the quality of the fit, including statistical measures. SpikeAnalyzer is equipped with an original pixel time correction algorithm, introduced to account for the exact time of acquisition of individual pixels of the object in the image. SpikeAnalyzer was tested to analyze both synthetic and raw confocal data.
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