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Next generation sequencing has opened new avenues for sequencing prokaryotes which were difficult or either impossible to culture. Now, it is possible to sequence all organisms present in a metagenomic community. However, a critical problem is to determine the host organism for each genomic fragment present in the dataset. This project provides the computational tools for assigning the organismal identity for each genomic scaffold in the given dataset.
For more detailed documentation please refer to the link below:
https://sourceforge.net/p/ngsscaffolds/wiki/Documentation/
Features
- Command-line Blast Analysis for binning scaffolds using sequence homology
- Calculation of tetranucleotide frequencies from test dataset
- Principal Component Analysis of scaffolds
- Command-line tool for selection of desired scaffolds from 3d graph of Principal Component Analysis
- %(G+C) Analysis
- Selection of desired scaffold peaks from %(G+C) graph
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